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easygo! human tnf-α one-step elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd easygo! human tnf-α one-step elisa kit
    Easygo! Human Tnf α One Step Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+elisa+kit/EasyGo!+Human+TNF-%CE%B1+One-Step+ELISA+Kit/custom%40ek182ega%4042641841
    Average 94 stars, based on 63 article reviews
    easygo! human tnf-α one-step elisa kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: IL-37 alleviates inflammatory effects and NLRP3 inflammasome activation in LPS-induced preterm birth
    Article Snippet: .. Supernatants collected from HTR-8/SVneo cells were assayed for IL-1β, IL-6, and TNF-α levels using a human ELISA kit (LiankeBio, Hangzhou, China). .. Intracellular IL-37 levels were measured using a human IL-37 ELISA kit (CUSABIO, Wuhan, China).

    Article Title: CD8 T Cell-Derived Exosomal miR-186-5p Elicits Renal Inflammation via Activating Tubular TLR7/8 Signal Axis.
    Article Snippet: After 24 h of cell culture, the HEK293T cells were treated with miRNA (5 μg ml−1) complexed with DOTAP for another 12 h and analyzed via luciferase (Promega). .. Enzyme-Linked Immunosorbent Assay: The concentration of cytokines including TNF-α and IL-6 in the cell culture supernatant was assessed by the human ELISA Kit (both from Multi Sciences) according to the manufacturer’s instructions. .. Cellular Fractionation Assay: The separation of nuclear and cytoplasmic fractions was performed using PARIS kits (Life Technologies, AM1921) according to the manufacturer’s instructions.

    Article Title: CD8 T Cell‐Derived Exosomal miR‐186‐5p Elicits Renal Inflammation via Activating Tubular TLR7/8 Signal Axis
    Article Snippet: After 24 h of cell culture, the HEK293T cells were treated with miRNA (5 μg ml −1 ) complexed with DOTAP for another 12 h and analyzed via luciferase (Promega). .. The concentration of cytokines including TNF‐ α and IL‐6 in the cell culture supernatant was assessed by the human ELISA Kit (both from Multi Sciences) according to the manufacturer's instructions. .. The separation of nuclear and cytoplasmic fractions was performed using PARIS kits (Life Technologies, AM1921) according to the manufacturer's instructions.

    Article Title: Immuno-transcriptomic analysis based on machine learning identifies immunity signature genes of chronic rhinosinusitis with nasal polyps.
    Article Snippet: .. The concentrations of IFN-γ, IL-4, IL-5, IL-13 and IL-17A in the supernatant were measured by Human ELISA Kit using an enzyme marker (PerkinElmer, Waltham, MS, USA) according to the manufacturer’s protocol (Multi Sciences, Guangzhou, China). .. The concentration of il-8 (Multi Sciences, Hangzhou, China) in the supernatant of HNEpCs cell cultures was measured by ELISA kit using an enzyme marker.

    Article Title: Systematic profiling of human gut bacteria with cyclic di-AMP secretion to enhance anti-tumor immunity.
    Article Snippet: THP-1 cells were seeded in a 6-well plate (5.5 × 105 cells per well) and cultured in 3 l g cyclic di-AMP (HY12326A, MCE) or within 200 lL volume of gut probiotic supernatant for 20 h, respectively. .. IFN-b levels were measured using a Human ELISA Kit (Multi Science), as described by the manufacturer. ..

    Article Title: sFRP5 ameliorates atherosclerosis by suppressing the JNK/TLR9 pathway in macrophages.
    Article Snippet: sFRP5 concentrations in serum samples was detected by the human sFRP5 EnzymeLinked Immunosorbent Assay (ELISA) kit (DY6266-05, R&D systems, United States) followed indications. .. Interleukin-6 (IL-6) concentrations in human serum samples were detected by human Elisa kit (EK106, Multi Sciences, China). .. Triglyceride (TG), total cholesterol (TC), and low-density lipoprotein C (LDL-C) of ApoE-/- mice were detected by assay kit (A111-1-1/A110-1-1/A113-1-1, Nanjing Jiancheng Bioengineering Institute, China).

    Article Title: Inflammatory Cytokines Changed in Patients With Depression Before and After Repetitive Transcranial Magnetic Stimulation Treatment
    Article Snippet: .. Serum levels of TNF-α, IFN-γ-, IL-4, IL-6, IL-8, IL-2, and CRP-hc for each sample were measured in duplicate using an enzyme-linked immunosorbent assay (ELISA) with a commercial human ELISA kit (Multisciences [Lianke] Biotech, Co., Ltd.), according to the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: CD8 T Cell-Derived Exosomal miR-186-5p Elicits Renal Inflammation via Activating Tubular TLR7/8 Signal Axis.
    Article Snippet: After 24 h of cell culture, the HEK293T cells were treated with miRNA (5 μg ml−1) complexed with DOTAP for another 12 h and analyzed via luciferase (Promega). .. Enzyme-Linked Immunosorbent Assay: The concentration of cytokines including TNF-α and IL-6 in the cell culture supernatant was assessed by the human ELISA Kit (both from Multi Sciences) according to the manufacturer’s instructions. .. Cellular Fractionation Assay: The separation of nuclear and cytoplasmic fractions was performed using PARIS kits (Life Technologies, AM1921) according to the manufacturer’s instructions.

    Article Title: CD8 T Cell‐Derived Exosomal miR‐186‐5p Elicits Renal Inflammation via Activating Tubular TLR7/8 Signal Axis
    Article Snippet: After 24 h of cell culture, the HEK293T cells were treated with miRNA (5 μg ml −1 ) complexed with DOTAP for another 12 h and analyzed via luciferase (Promega). .. The concentration of cytokines including TNF‐ α and IL‐6 in the cell culture supernatant was assessed by the human ELISA Kit (both from Multi Sciences) according to the manufacturer's instructions. .. The separation of nuclear and cytoplasmic fractions was performed using PARIS kits (Life Technologies, AM1921) according to the manufacturer's instructions.

    Cell Culture:

    Article Title: CD8 T Cell-Derived Exosomal miR-186-5p Elicits Renal Inflammation via Activating Tubular TLR7/8 Signal Axis.
    Article Snippet: After 24 h of cell culture, the HEK293T cells were treated with miRNA (5 μg ml−1) complexed with DOTAP for another 12 h and analyzed via luciferase (Promega). .. Enzyme-Linked Immunosorbent Assay: The concentration of cytokines including TNF-α and IL-6 in the cell culture supernatant was assessed by the human ELISA Kit (both from Multi Sciences) according to the manufacturer’s instructions. .. Cellular Fractionation Assay: The separation of nuclear and cytoplasmic fractions was performed using PARIS kits (Life Technologies, AM1921) according to the manufacturer’s instructions.

    Article Title: CD8 T Cell‐Derived Exosomal miR‐186‐5p Elicits Renal Inflammation via Activating Tubular TLR7/8 Signal Axis
    Article Snippet: After 24 h of cell culture, the HEK293T cells were treated with miRNA (5 μg ml −1 ) complexed with DOTAP for another 12 h and analyzed via luciferase (Promega). .. The concentration of cytokines including TNF‐ α and IL‐6 in the cell culture supernatant was assessed by the human ELISA Kit (both from Multi Sciences) according to the manufacturer's instructions. .. The separation of nuclear and cytoplasmic fractions was performed using PARIS kits (Life Technologies, AM1921) according to the manufacturer's instructions.

    Marker:

    Article Title: Immuno-transcriptomic analysis based on machine learning identifies immunity signature genes of chronic rhinosinusitis with nasal polyps.
    Article Snippet: .. The concentrations of IFN-γ, IL-4, IL-5, IL-13 and IL-17A in the supernatant were measured by Human ELISA Kit using an enzyme marker (PerkinElmer, Waltham, MS, USA) according to the manufacturer’s protocol (Multi Sciences, Guangzhou, China). .. The concentration of il-8 (Multi Sciences, Hangzhou, China) in the supernatant of HNEpCs cell cultures was measured by ELISA kit using an enzyme marker.



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    Image Search Results


    Substrate characterisation in 2D and 3D cell culture—biological responses and physical properties with different compositions. (a) hPSC confluence in 2D growth factor-reduced Matrigel (Geltrex), laminin 521, fibrin-laminin hydrogel, and fibrin gel after 4 d; scale bar = 100 μ m. (b) hPSCs cultured in 3D hydrogels. Top panel: fibrin (5 mg ml −1 ) gel. Bottom panel: Alphagel containing structures resembling pluripotent spheroids; scale bar = 200 μ m. Scanning electron microscopy of (c) fibrin gel and (d) Alphagel; scale bar = 1 μ m, magnification 20 K X, iProbe = 13 pA, 2.00 kV, Working Distancee = 4.4 mm for both images. (e) Young’s moduli in hydrogels with varying fibrin and laminin concentrations. One-way ANOVA; ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001. (f) Cell viability of hPSCs cultured in Alphagel, fibrin-only hydrogels, and 2D standard substrates. Mean ± sandard error of the mean displayed. t -test; * = p < 0.05. (g) ELISA of laminin 521 in culture media used with acellular Alphagel and (h) the calculated amount of fibrin-bound laminin.

    Journal: Materials Futures

    Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine

    doi: 10.1088/2752-5724/ae4e4d

    Figure Lengend Snippet: Substrate characterisation in 2D and 3D cell culture—biological responses and physical properties with different compositions. (a) hPSC confluence in 2D growth factor-reduced Matrigel (Geltrex), laminin 521, fibrin-laminin hydrogel, and fibrin gel after 4 d; scale bar = 100 μ m. (b) hPSCs cultured in 3D hydrogels. Top panel: fibrin (5 mg ml −1 ) gel. Bottom panel: Alphagel containing structures resembling pluripotent spheroids; scale bar = 200 μ m. Scanning electron microscopy of (c) fibrin gel and (d) Alphagel; scale bar = 1 μ m, magnification 20 K X, iProbe = 13 pA, 2.00 kV, Working Distancee = 4.4 mm for both images. (e) Young’s moduli in hydrogels with varying fibrin and laminin concentrations. One-way ANOVA; ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001. (f) Cell viability of hPSCs cultured in Alphagel, fibrin-only hydrogels, and 2D standard substrates. Mean ± sandard error of the mean displayed. t -test; * = p < 0.05. (g) ELISA of laminin 521 in culture media used with acellular Alphagel and (h) the calculated amount of fibrin-bound laminin.

    Article Snippet: Apolipoprotein B (APOB) secretion in the supernatant was quantified using the human APOB ELISA quantification kit (Mabtech, no. 3715-1H-6) according to the product literature.

    Techniques: Cell Culture, Electron Microscopy, Enzyme-linked Immunosorbent Assay

    Characterisation of iHeps derived in Alphagel, fibrin-only hydrogels, and Matrigel. (a) Key hepatocyte markers in Alphagel-derived iHeps. ALB = albumin, HNF = hepatocyte nuclear factor, CYP2A6 = Cytochrome P450 2A6, CD147 = cluster of differentiation protein 147, and E-CAD = E-cadherin. Scale bar = 25 μ m. (b) Key hepatocyte markers by gene expression (qPCR): CCAAT/enhancer-binding protein alpha (CEBPA), T-box transcription factor 3= TBX3, alpha-fetoprotein = AFP. (c) Albumin secretion (ELISA) and (d) CYP3A4 activity (P450-Glo TM ) in PHHs versus iHeps cultured in various gels (day 22). HCM = Hepatocyte Culture Media (Lonza). (e) LDL uptake (red) in Alphagel-derived iHeps versus hPSCs. Scale bar = 100 μ m. (f) CDFDA secretion (green) in Alphagel-derived iHeps versus hPSCs. Top panel scale bar = 20 μ m; bottom panel scale bar = 50 μ m. One-way ANOVA was used; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001.

    Journal: Materials Futures

    Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine

    doi: 10.1088/2752-5724/ae4e4d

    Figure Lengend Snippet: Characterisation of iHeps derived in Alphagel, fibrin-only hydrogels, and Matrigel. (a) Key hepatocyte markers in Alphagel-derived iHeps. ALB = albumin, HNF = hepatocyte nuclear factor, CYP2A6 = Cytochrome P450 2A6, CD147 = cluster of differentiation protein 147, and E-CAD = E-cadherin. Scale bar = 25 μ m. (b) Key hepatocyte markers by gene expression (qPCR): CCAAT/enhancer-binding protein alpha (CEBPA), T-box transcription factor 3= TBX3, alpha-fetoprotein = AFP. (c) Albumin secretion (ELISA) and (d) CYP3A4 activity (P450-Glo TM ) in PHHs versus iHeps cultured in various gels (day 22). HCM = Hepatocyte Culture Media (Lonza). (e) LDL uptake (red) in Alphagel-derived iHeps versus hPSCs. Scale bar = 100 μ m. (f) CDFDA secretion (green) in Alphagel-derived iHeps versus hPSCs. Top panel scale bar = 20 μ m; bottom panel scale bar = 50 μ m. One-way ANOVA was used; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001.

    Article Snippet: Apolipoprotein B (APOB) secretion in the supernatant was quantified using the human APOB ELISA quantification kit (Mabtech, no. 3715-1H-6) according to the product literature.

    Techniques: Derivative Assay, Gene Expression, Binding Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Cell Culture

    Characterisation of iHeps cultured in Hepatogel and its effect on cell retention after intra-hepatic cell transplantation. (a) Differentially expressed genes in iHeps: Hepatologel, Alphagel, Matrigel, and adult PHHs. (b) A heat map summarising the differential gene expression of a hepatic 24-gene panel across replicates of Matrigel, Alphagel, and Hepatogel (normalised to hPSC). (c) Albumin ELISA of culture media and (d) luciferin-based measure of CYP3A4 activity: 2 d after completion of iHep differentiation and 2 d after plating PHHs. One-way ANOVA; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001. (e) Mouse livers 3 d after intra-hepatic injection with H-iHeps in Hepatogel and 0.9% saline. Red box = area magnified. * = site of injection, dotted white lines demarcate engrafted cell mass. Scale bar = 1 mm. (f) Human albumin (stained red) in engrafted iHeps 3 d after intra-hepatic injection. Scale bar = 100 μ m. (g) H-iHeps identified by albumin staining on liver histology 3 d after intra-hepatic injection. (h) ELISA of mouse serum for human albumin after injection with H-iHeps in Hepatogel and 0.9% saline over time. Day 0 = serum levels before injection. T -test; *** = p < 0.001 and **** = p < 0.0001.

    Journal: Materials Futures

    Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine

    doi: 10.1088/2752-5724/ae4e4d

    Figure Lengend Snippet: Characterisation of iHeps cultured in Hepatogel and its effect on cell retention after intra-hepatic cell transplantation. (a) Differentially expressed genes in iHeps: Hepatologel, Alphagel, Matrigel, and adult PHHs. (b) A heat map summarising the differential gene expression of a hepatic 24-gene panel across replicates of Matrigel, Alphagel, and Hepatogel (normalised to hPSC). (c) Albumin ELISA of culture media and (d) luciferin-based measure of CYP3A4 activity: 2 d after completion of iHep differentiation and 2 d after plating PHHs. One-way ANOVA; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001. (e) Mouse livers 3 d after intra-hepatic injection with H-iHeps in Hepatogel and 0.9% saline. Red box = area magnified. * = site of injection, dotted white lines demarcate engrafted cell mass. Scale bar = 1 mm. (f) Human albumin (stained red) in engrafted iHeps 3 d after intra-hepatic injection. Scale bar = 100 μ m. (g) H-iHeps identified by albumin staining on liver histology 3 d after intra-hepatic injection. (h) ELISA of mouse serum for human albumin after injection with H-iHeps in Hepatogel and 0.9% saline over time. Day 0 = serum levels before injection. T -test; *** = p < 0.001 and **** = p < 0.0001.

    Article Snippet: Apolipoprotein B (APOB) secretion in the supernatant was quantified using the human APOB ELISA quantification kit (Mabtech, no. 3715-1H-6) according to the product literature.

    Techniques: Cell Culture, Transplantation Assay, Gene Expression, Enzyme-linked Immunosorbent Assay, Activity Assay, Injection, Saline, Staining